1612 Non-invasive method for comprehensive skin whole genome methylome mapping

Aging and sun exposure are the leading causes of skin cancer. It has been shown that epigenetic changes, such as DNA methylation, well established mechanisms for However, large scale data acquisition is a problem it relies mostly on invasive biopsies. Here we present method non-invasive sample collection using tape-stripping analysis. We compared matched shave biopsies tape stripping samples to evaluate feasibility validation reproducibility in sun-protected sun-exposed from cohort covering various ages, ethnicities types (n=277) enzymatic conversion whole genome Illumina sequencing. show good correlation global methylation landscape between biopsy. Moreover, consistent profiles collected different parts body (FDR < 1e −5). identify hypomethylated blocks healthy epidermis result chronic UV irradiation. Results also indicate significant age-related hypermethylation CpG islands. Interestingly, this effect was strongly enriched at specific age window, 50–55 years. These describe combined with sequencing way measure changes suggest tape-stripping, particularly when done cohorts patients, could be used biomarkers ageing damage, possibly track treatment response interventions.